circRNA sequencing
 

High throughput sequencing was studied for the circular RNA and circRNA function.

 
Sample Type
total RNA
 
Sample Volume
≥ 10 μg
 
Delivery cycle
70 Days (Urgent service available)

Service Introduction

 

Circular RNAs (circRNAs) are currently classed as non-coding RNA (ncRNA) that, unlike linear RNAs, form covalently closed continuous loops and act as gene regulators in mammals.It has been proposed that circRNA regulate gene expression at the transcriptional or post-transcriptional level by interacting with miRNAs and that circRNAs may have a role in regulating miRNA function in cancer initiation and progression.

Workflow

 

Sample QC

Library construction

Sequencing

Data QC

Data analysis

Parameters

 

Platform

HiSeq,PE150

Data

10 G

Data analysis

 
1

Reads QC/QA

2

circRNA identification analysis

3

circRNA quantification analysis

4

circRNA diffirential expression analysis

5

CircRNA gene enrichment analysis

6

miRNA binding site analysis

Immediate consultation or appointment testing services
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