sRNA sequencing
 

Using NGS for specific organizations in a particular state small RNA

 
Sample Type
total RNA
 
Sample Volume
≥ 2 μg
 
Delivery cycle
45 Days (Urgent service available)

Service Introduction

 

Small RNA are<200 nt (nucleotide) in length, and are usually non-coding RNA molecules.RNA silencing is often a function of these molecules, with the most common and well-studied example being RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA).        

Workflow

 

Sample QC

Library construction

Sequencing

Data QC

Data analysis

Parameters

 

Platform

HiSeq

Data

10~20 M clean reads

Data analysis

 
1

Reads QC/QA

2

Public sequence and specific sequence analysis

3

Distribution of Srna in selected reference genome

4

miRNA screening

5

miRNA prediction

6

Classified annotation

7

Targeted gene prediction

8

Targeted transcript analysis

Immediate consultation or appointment testing services
Please select
  • 受检者
  • 研究人员
  • 医生
  • 公司
  • 其他

You can dial400-158-5999 The hotline contact us, or fill in your contact information and concerns, we will contact you in time