Whole genome methylation sequencing
 

Using high-throughput sequencing for accurate methylation within the scope of whole genome research.

 
Sample Type
DNA sample
 
Sample Volume
≥ 2 μg
 
Delivery cycle
90 Days (Probe design not included, Urgent service available)

Service Introduction

 

DNA methylation at the C5 position of cytosine plays a crucial role in gene expression and chromatin remodelling, and perturbations in methylation patterns are implicated in the development of cancer, neurodegenerative diseases, and neurological disorders. Therefore, the mapping of methylated bases (the methylome) is critical to understanding gene expression and other processes subject to epigenetic regulation.

Workflow

 

Sample QC

Library construction

Sequencing

Data QC

Data analysis

Parameters

 

Platform

HiSeq,PE150

Data

90 GB

Data analysis

 
1

Base calling, raw data clean up and QC in pipeline.

2

Reference genome contrast.

3

Analyze methylation level, coverage, distribution of C base.

4

DMS, DMR and DMP analysis.

5

Functional enrichment analysis of DMR and DMP

Immediate consultation or appointment testing services
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You can dial400-158-5999 The hotline contact us, or fill in your contact information and concerns, we will contact you in time