LncRNA sequencing
 

Using NGS for long non-coding RNAs (LncRNAs) are transcribed RNAs longer than 200 nt that do not encode for proteins.

 
Sample Type
total RNA
 
Sample Volume
≥ 2 μg
 
Delivery cycle
60 Days (Urgent service available)

Service Introduction

 

Long non-coding RNAs (LncRNAs) are transcribed RNAs longer than 200 nt that do not encode for proteins. LncRNAs are involved in a wide range of important cellular processes such as X-chromosome inactivation, imprinting, maintenance of pluripotency, lineage commitment and apoptosis. LncRNAs are also known to be involved with human diseases such as cancer, cardiovascular disease and neurological disorders, and are of significant interest to researchers.

Workflow

 

Sample QC

Library construction

Sequencing

Data QC

Data analysis

Parameters

 

Platform

HiSeq,PE150

Data

12G or more

Data analysis

 
1

Reads QC/QA

2

Reference genome contrast

3

Known expression level analysis

4

Diffirential expression analysis

5

Diffirential expressed gene GO and KEGG analysis

6

Alternative splicing, new transcript, target prediction.

7

New lncRNA gene prediction

8

lncRNA targeted gene prediction

9

Integrated analysis of lncRNA and mRNA

Immediate consultation or appointment testing services
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